what is immunoassay
the definition is A laboratory technique that makes use of the binding between an antigen and its homologous antibody in order to identify and quantify the specific antigen or antibody in a sample.
source: http://education.yahoo.com/reference/dictionary/entry/immunoassay
how is it screen?
it is screen by using a probe. instead of a probe, an antibody that binds to the protein encoded by the target gene is used. the steps are similar to prbe hybridization except that the proteins are crucial to the immunological assay. hence, after lysis of teh cells, the matrix and proteins are treated with the antibody (primary antibody). after incubation, unbound antibody is washed away and another antibody (secondy antibody) specific for the primary antibody is applied. for visualization, an enzyme may be attached to the secondary antibody whose substarte is colourless. upon introduction of the substrate, the enzyme hydrolyses it to produce a coloured compound at the site of reaction, thereby identifying the clone that may harbor tha target DNA.
what it detect and the method format?
it can help to detect foodborne viruses, dependent on the inherent ability of living systems to produce antobodies against foreign substances(antigens) which are specific for that antigen. it is the specificity that makes it useful for diagnostic tool. these antigens can be proteins, lipid constituents or nucleic acids of a virus, as lipid constituent is host derived, it is not useful as a means for detecting teh virus.
the availble methods formats are: enzyme-linked immunosorbent assay(ELISA), radioimmunoassay(RIA), immunodiffusion, immnoblotting, latex agglutination(LA), and countercurrent immunoelectrophoresis(CIE). many of these methods have been aplied to detect human enteric viruses in clinical specimens and for detection of plant viruses in food crops. the methods used are not specific to foodborne agents and are described in standard manuals.
immunoassays are ease of use, relative low cost, and feasibility of testing large numbers of specimens rapidly. however, they are insensitive when compared to culture and gene amplification methods. and they do not differentiation between 'dead' antigen and viable infectious organisms.
source:
book name:
george acguaah. (2004) understanding biotechnology. published: pearson education (USA)
source: http://education.yahoo.com/reference/dictionary/entry/immunoassay
how is it screen?
it is screen by using a probe. instead of a probe, an antibody that binds to the protein encoded by the target gene is used. the steps are similar to prbe hybridization except that the proteins are crucial to the immunological assay. hence, after lysis of teh cells, the matrix and proteins are treated with the antibody (primary antibody). after incubation, unbound antibody is washed away and another antibody (secondy antibody) specific for the primary antibody is applied. for visualization, an enzyme may be attached to the secondary antibody whose substarte is colourless. upon introduction of the substrate, the enzyme hydrolyses it to produce a coloured compound at the site of reaction, thereby identifying the clone that may harbor tha target DNA.
what it detect and the method format?
it can help to detect foodborne viruses, dependent on the inherent ability of living systems to produce antobodies against foreign substances(antigens) which are specific for that antigen. it is the specificity that makes it useful for diagnostic tool. these antigens can be proteins, lipid constituents or nucleic acids of a virus, as lipid constituent is host derived, it is not useful as a means for detecting teh virus.
the availble methods formats are: enzyme-linked immunosorbent assay(ELISA), radioimmunoassay(RIA), immunodiffusion, immnoblotting, latex agglutination(LA), and countercurrent immunoelectrophoresis(CIE). many of these methods have been aplied to detect human enteric viruses in clinical specimens and for detection of plant viruses in food crops. the methods used are not specific to foodborne agents and are described in standard manuals.
immunoassays are ease of use, relative low cost, and feasibility of testing large numbers of specimens rapidly. however, they are insensitive when compared to culture and gene amplification methods. and they do not differentiation between 'dead' antigen and viable infectious organisms.
source:
book name:
george acguaah. (2004) understanding biotechnology. published: pearson education (USA)
posted by .:: Estelle ::. @ 12:39
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